This team uses Competitive-ELISA as a method. It can detect neomycin (Neo) in samples, such as muscle, raw milk, etc. This kit consists of an ELISA microtiter plate, HRP conjugate, antibody working solution, standard, and other supplemental reagents.
The microtiter plate of this kit has been precoated with coupled antigen. During the reaction, Neo in the samples or standard competes with the antigen attached to the solid phase support for the anti-Neo antibody sites.
Then, Horseradish Peroxidase Conjugate (HRP) is added to each well of the microtiter plate and a Substrate Reagent for Color Development is added. There is a negative correlation between the OD value of the samples and the Neo concentration. The concentration of Neo in the samples can be calculated by comparing the OD of the samples with the standard curve.
Sensitivity: 0.5 ppb (ng/mL)
Reaction mode: 25℃, 30 min~ 15 min
Detection limit: Muscle, Egg —50 ppb, Raw milk —20 ppb
Cross-reactivity: Neomycin–100%, Streptomycin, Kanamycin, Gentamicin—<0.1%,
Sample recovery rate: 90%±30%.
|ELISA Microtiter Plate||96 wells|
|Standard Liquid||1 mL each
(0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb)
|HRP Conjugate||7 mL|
|Antibody Working Solution||7 mL|
|Substrate Reagent A||6 mL|
|Substrate Reagent B||6 mL|
|Stop Solution||6 mL|
|20×Concentrated Wash Buffer||25 mL|
|5×Concentrated Sample Solution||30 mL|
|Plate Sealer||3 pieces|
|Sealed Bag||1 piece|
Other supplies required
Instruments: Microplate reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g).
Micropipette: Single channel (20-200 μL, 100-1000 μL), Multichannel (30-300 μL).
Reagents: Trichloroacetic Acid (C2HCl3O2), Concentrated H2SO4, NaCl.
Storage and expiry date
Store the kit at 2~8℃. Do not freeze any test kit components.
Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 – 8℃.