Neo(Neomycin) ELISA Kit

This team uses Competitive-ELISA as a method. It can detect neomycin (Neo) in samples, such as muscle, raw milk, etc. This kit consists of an ELISA microtiter plate, HRP conjugate, antibody working solution, standard, and other supplemental reagents.

The microtiter plate of this kit has been precoated with coupled antigen. During the reaction, Neo in the samples or standard competes with the antigen attached to the solid phase support for the anti-Neo antibody sites.

Then, Horseradish Peroxidase Conjugate (HRP) is added to each well of the microtiter plate and a Substrate Reagent for Color Development is added. There is a negative correlation between the OD value of the samples and the Neo concentration. The concentration of Neo in the samples can be calculated by comparing the OD of the samples with the standard curve.

Technical indicator

Sensitivity: 0.5 ppb (ng/mL)

Reaction mode: 25℃, 30 min~ 15 min

Detection limit: Muscle, Egg —50 ppb, Raw milk —20 ppb

Cross-reactivity: Neomycin–100%, Streptomycin, Kanamycin, Gentamicin—<0.1%,

Sample recovery rate: 90%±30%.

Kits components

Item Specifications
ELISA Microtiter Plate 96 wells
Standard Liquid 1 mL each

(0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb)

HRP Conjugate 7 mL
Antibody Working Solution 7 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20×Concentrated Wash Buffer 25 mL
5×Concentrated Sample Solution 30 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy

Other supplies required

Instruments: Microplate reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g).

Micropipette: Single channel (20-200 μL, 100-1000 μL), Multichannel (30-300 μL).

Reagents: Trichloroacetic Acid (C2HCl3O2), Concentrated H2SO4, NaCl.

Storage and expiry date

Store the kit at 2~8℃. Do not freeze any test kit components.

Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 – 8℃.

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