This kit uses Competitive-ELISA as the method. It can detect estradiol (E2) in samples, such as meat, milk, feed, etc. This kit consists of an ELISA microtiter plate, HRP conjugate, antibody working solution, standard, and other supplemental reagents.
The microtiter plate of this kit has been precoated with coupled antigen. During the reaction, E2 in the samples or standard competes with the antigen attached to the solid phase support for the anti-E2 antibody sites.
Then, the Horseradish Peroxidase Conjugate (HRP) is added to each well of the microtiter plate and the Substrate Reagent for Color Development is added. There is a negative correlation between the OD value of the samples and the E2 concentration. The E2 concentration in the samples can be calculated by comparing the OD of the samples with the standard curve.
Sensitivity: 0.1 ppb (ng / mL)
Reaction mode: 25 ℃, 30 min ~ 15 min
Detection limit: meat, milk, feed, milk powder — 5 ppb
Cross reactivity: estradiol — 100%; Estriol, hexestrol — <1%
Sample recovery rate: 90% ± 30%.
|ELISA Microtiter plate||96 wells|
|Standard Liquid||1 mL each(0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb)|
|Antibody Working Solution||7 mL|
|HRP Conjugate||12 mL|
|Sample Diluent||50 mL|
|Substrate Reagent A||6 mL|
|Substrate Reagent B||6 mL|
|Stop Solution||6 mL|
|20×Concentrated Wash Buffer||25 mL|
|Plate Sealer||3 pieces|
|Sealed Bag||1 piece|
Other materials required but not supplied
Instrument: Microplate Reader, Homogenizer, Oscillators, Nitrogen Evaporators, Water Bath, Centrifuge, Balance (sensitivity 0.01 g).
Micropipette: single channel (20-200 μL, 100-1000 μL), multichannel (300 μL).
Reagents: Acetonitrile, Acetone, NaOH, H3PO4 (≥85.0%), Trichloromethane.
Storage and expiration date
Store the kit at 2 ~ 8 ℃. Do not freeze any component of the test kit.
Return unused microwells to their original foil pouch and reseal together with the supplied desiccant and store at 2-8 ° C.